CARM1 antibody (AA 200-400)

Catalog No. ABIN7859467

The Mouse Monoclonal anti-CARM1 antibody (Clone CARM1-7426) (ABIN7859467) specifically detects CARM1 in IHC. The antibody is reactive with Human samples.

Quick Overview for CARM1 antibody (AA 200-400) (ABIN7859467)

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CARM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CARM1-7426

Product Details anti-CARM1 Antibody

Binding Specificity: AA 200-400

Purpose: CARM1 Antibody

Specificity: CARM1 (coactivator-associated arginine methyltransferase 1), also known as protein arginine N-methyltransferase 4 (PRMT4), is a 585 amino acid nuclear and cytoplasmic protein belonging to the protein arginine N-methyltransferase family. As a protein arginine N-methyltransferase, CARM1 is capable of catalyzing the transfer of methyl groups from S-adenosylmethionine to the guanidino group nitrogen atoms of arginine residues in certain proteins involved in mRNA stability, DNA packaging and transcriptional regulation. The methyltransferase activity of CARM1 has been found to be negatively regulated through phosphorylation at a conserved serine residue. CARM1 acts as a positive regulator for multiple transcription factors and functions as a secondary coactivator through its association with p160 coactivators. CARM1 exists as two alternatively spliced isoforms, and is encoded by a gene that maps to human chromosome 19p13.2.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa200-400) of human CARM1 protein (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CARM1

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Alternative Name: CARM1

Background:

EF-hand calcium-binding protein 1, EFCBP1, N-terminal EF-hand calcium-binding protein 1, Neuronal calcium-binding protein 1, STIP-1, synaptotagmin interacting protein 1

Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Molecular Weight: 64/45 kDa (isoform 1 / isoform 2)

Gene ID: 10498, 323213

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation