CD27 antibody (AA 28-170)

Catalog No. ABIN7863617

The Mouse Monoclonal anti-CD27 antibody is suitable to detect CD27 in samples from Human. It has been validated for WB and IHC.

Quick Overview for CD27 antibody (AA 28-170) (ABIN7863617)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: LPFS2-4177

Product Details anti-CD27 Antibody

Binding Specificity: AA 28-170

Purpose: CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody

Specificity: Recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NF B and SAPK/JNK and induces apoptosis.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background:

LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7

Raji, Ramos cells. Human peripheral blood lymphocytes. Human tonsil and stomach tissues.

Molecular Weight: 120kDa

Gene ID: 939, 355307

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints