EBI3 antibody (AA 366-466)

Catalog No. ABIN7864983

This Mouse Monoclonal antibody specifically detects EBI3 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for EBI3 antibody (AA 366-466) (ABIN7864983)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: EBI3-8902

Product Details anti-EBI3 Antibody

Binding Specificity: AA 366-466

Purpose: EBI3 / Epstein-Barr virus induced 3 Antibody

Specificity: Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27). IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells. Interferon-b differentially regulates expression of the IL-12 family members p35, p40, p19 and Ebi3 in activated human dendritic cells. Ebi3 may function to antagonize IL-12 and to inhibit the development of a Th1 immune response. Ebi3 is strongly expressed in Hodgkin and Reed-Sternberg cells, independently of the EBV status of the tumor cells. Research suggests that Ebi3 may be an additional component of the repertoire employed by Hodgkin and Reed-Sternberg cells to inhibit an effective anti-tumor or anti-viral immune response. The human Ebi3 gene maps to chromosome 19p13.3 and encodes a secreted glycoprotein that is expressed in spleen and tonsils, and at high levels in full-term placenta.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 366-466) of human EBI3 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: EBI3

Background:

cytokine receptor, EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, Epstein Barr virus induced gene 3, Epstein Barr virus induced gene 3 protein, IL27 subunit beta, IL27B, IL35 subunit, interleukin 27 subunit beta

Human placenta.

Molecular Weight: 34 kDa

Gene ID: 10148, 501452

UniProt: Q14213