Recombinant PD-L1 antibody (AA 39-191)

Catalog No. ABIN7862918

This Rabbit Monoclonal antibody specifically detects PD-L1 in WB, FACS, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant PD-L1 antibody (AA 39-191) (ABIN7862918)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PDL1-4451R

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 39-191

Purpose: Recombinant PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody

Specificity: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background:

B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1

Human heart, Placenta, RAW or HepG2 cells., spleen or tonsil tissue. Jurkat, A549, MDA-MB-231, Raw.

Molecular Weight: 37-50kDa

Gene ID: 29126, 521989

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints