Recombinant Cytokeratin 5 antibody (AA 390-590)

Catalog No. ABIN7863807

The Mouse Monoclonal anti-Cytokeratin 5 antibody is suitable to detect Cytokeratin 5 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant Cytokeratin 5 antibody (AA 390-590) (ABIN7863807)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RKRT5-6969

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: AA 390-590

Purpose: Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody

Specificity: This Mab recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, yoepithelial, and mesothelium.Ç 79oΩAnti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Ç 79oΩAnti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.Ç 79oΩ

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 390-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5

Background:

58 kDa Cytokeratin, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, Type-II Cytoskeletal 5, Type-II keratin Kb5

esophagus or bladder tissue (IHC).|Human tonsil

Molecular Weight: 58kDa

Gene ID: 3852, 433845

UniProt: P13647