Recombinant P-Glycoprotein antibody (AA 500-700)

Catalog No. ABIN7860324

The Rabbit Monoclonal anti-P-Glycoprotein antibody is suitable to detect P-Glycoprotein in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant P-Glycoprotein antibody (AA 500-700) (ABIN7860324)

Target: P-Glycoprotein

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This P-Glycoprotein antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MDR1-8962R

Product Details anti-P-Glycoprotein Antibody

Binding Specificity: AA 500-700

Purpose: Recombinant P-glycoprotein / p170 / MDR1 / CD243 Antibody

Specificity: Cells selected for resistance to a single cytotoxic drug may become crossresistant to a broad range of drugs with different structures and cellular targets. This phenomenon is called multiple drug resistance (MDR). MDR proteins (Mdrs) are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr-1 is an apical transmembrane protein that is an integral part of the blood-brain barrier and functions as a drug-transport pump transporting a variety of drugs from the brain back into the blood. The Mdr-1 gene is known as ABCB1 and is located on human chromosome 7q21.12. The mouse homolog of Mdr-1 is known as Mdr-3. Interestingly, a murine protein by the name of Mdr-1 exists and is encoded by the murine Abcb1b gene, but it is not homologous with human Mdr-1.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa500-700) of human ABCB1 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for P-Glycoprotein

Target: P-Glycoprotein

Background:

ABC20, ABCB1, ATP-binding cassette sub-family B member 1, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170, Multidrug resistance protein 1 (MDR1), P-glycoprotein 1 (PGY1)

hPBL or Jurkat whole cell lysate. Human adrenal gland.

Molecular Weight: 170kDa

Gene ID: 5243, 489033

UniProt: P08183