PIK3CD antibody (AA 520-720)

Catalog No. ABIN7865035

The Mouse Monoclonal anti-PIK3CD antibody is suitable to detect PIK3CD in samples from Human. It has been validated for WB and IHC.

Quick Overview for PIK3CD antibody (AA 520-720) (ABIN7865035)

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3CD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: PIK3CD-4639

Product Details anti-PIK3CD Antibody

Binding Specificity: AA 520-720

Purpose: PIK3CD Antibody

Specificity: Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85a and p85b), each possessing one SH3 and two SH2 domains. Various p110 forms have been identified. p110a and p110b interact with p85a, and p110a has also been shown to interact with p85b in vitro. It has been shown to bind p85a and b, but it apparently does not phosphorylate these subunits. p110d has the capacity to autophosphorylate and results in the nearly complete inactivation of the lipid kinase activity. Interestingly, p110g does not interact with the p85 subunits and has been shown to be activated by a and bg heterotrimeric G proteins. Two p110d isoforms have been identified and are widely expressed. Isoform 1 is expressed predominantly in leukocytes while isoform 2 is expressed in normal thymus, lung and spleen tissues.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human PIK3CD protein (around aa 520-720) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for PIK3CD

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Alternative Name: PIK3CD

Background:

5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, APDS, GRB1, IMD14, p110delta, p110dp85a, p85-ALPHA, Phosphoinositide 3 kinase B, Phosphoinositide 3 kinase C, Phosphoinositide 3 kinase catalytic delta polypeptide, Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta, PI3K-delta, PIK3R1, PtdIns 3 kinase p110, PtdIns 3 kinase subunit p110 delta, PtdIns-3-kinase subunit delta, PtdIns-3-kinase subunit p110-delta

lymph node or tonsil|THP1 cells. Human spleen

Molecular Weight: 119/33kDa

Gene ID: 5293, 518451

UniProt: O00329

Pathways: BCR Signaling, Warburg Effect