Recombinant FTH1 antibody (AA 58-180)

Catalog No. ABIN7860399

The Rabbit Monoclonal anti-FTH1 antibody is suitable to detect FTH1 in samples from Human and Rat. It has been validated for WB and IHC.

Quick Overview for Recombinant FTH1 antibody (AA 58-180) (ABIN7860399)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: FTH-8700R

Product Details anti-FTH1 Antibody

Binding Specificity: AA 58-180

Purpose: Recombinant Ferritin, Heavy Chain (FTH) (Microglia Marker) Antibody

Specificity: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: Ferritin, Heavy Chain

Background:

Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF)

Human liver tissue. Liver tissue lysate, A-549, A-431, Human Brain, Rat Brain, Human Kidney, Rat Kidney, Human Lungs, Rat Lungs

Molecular Weight: 21kDa

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis