alpha 2 Macroglobulin antibody (AA 600-800)

Catalog No. ABIN7860442

The Mouse Monoclonal anti-alpha 2 Macroglobulin antibody (Clone A2M-6550) (ABIN7860442) specifically detects alpha 2 Macroglobulin in IHC. The antibody is reactive with Human samples.

Quick Overview for alpha 2 Macroglobulin antibody (AA 600-800) (ABIN7860442)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: A2M-6550

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: AA 600-800

Purpose: Alpha-2-Macroglobulin Antibody

Specificity: ±-2-Macroglobulin (±-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, ±-2M was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on ±-2M. This interaction induces a conformational change in ±-2M, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, ±-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor ≤ (TGF≤) in serum is primarily bound to ±-2M, which renders TGF≤ inactive. ±-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding ±-2M are associated with an increased incidence of Alzheimer s disease (AD), which is consistent with the role of ±-2M in mediating the clearance and degradation of A ≤, the major component of ≤-Amyloid deposits accumulated during AD.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human recombinant A2M protein fragment (around aa 600-800) (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: Alpha-2-Macroglobulin

Background:

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, Alpha-2-M (A2M), CPAMD5

Human liver or placenta tissues.

Molecular Weight: 185kDa

Gene ID: 2, 212838

UniProt: P01023

Pathways: Lipid Metabolism