alpha 2 Macroglobulin antibody (AA 604-748)

Catalog No. ABIN7864202

The Mouse Monoclonal anti-alpha 2 Macroglobulin antibody is suitable to detect alpha 2 Macroglobulin in samples from Human. It has been validated for IHC.

Quick Overview for alpha 2 Macroglobulin antibody (AA 604-748) (ABIN7864202)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: A2M-4849

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: AA 604-748

Purpose: Alpha-2-Macroglobulin / A2M Antibody

Specificity: α-2-Macroglobulin (α-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, α-2M was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on α-2M. This interaction induces a conformational change in α-2M, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, α-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor β (TGFβ) in serum is primarily bound to α-2M, which renders TGFβ inactive. α-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding α-2M are associated with an increased incidence of Alzheimer s disease (AD), which is consistent with the role of α-2M in mediating the clearance and degradation of A β, the major component of β-Amyloid deposits accumulated during AD.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human recombinant A2M protein fragment (around aa 604-748) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: Alpha-2-Macroglobulin

Background:

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, Alpha-2-M (A2M), CPAMD5

Human liver or placenta tissues.

Molecular Weight: 185kDa

Gene ID: 2, 212838

UniProt: P01023

Pathways: Lipid Metabolism