Recombinant ERG antibody (C-Term)

Catalog No. ABIN7862753

The Rabbit Monoclonal anti-ERG antibody (Clone ERG-22R) (ABIN7862753) specifically detects ERG in WB and IHC. The antibody is reactive with Human, Mouse, Guinea Pig and Hamster samples.

Quick Overview for Recombinant ERG antibody (C-Term) (ABIN7862753)

Target: ERG (ERG, ETS transcription factor (ERG))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Guinea Pig, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ERG-22R

Product Details anti-ERG Antibody

Binding Specificity: C-Term

Purpose: Recombinant ERG / ETS Transcription Factor (Prostate Cancer Marker) Antibody

Specificity: ERG (ETS-related gene) is a proto-oncogene, a member of the ETS family of transcription factors. The ERG gene encodes for a nuclear protein, also called ERG, which is involved in hematopoietic and endothelial development. ERG remains constitually expressed in endothelial cells in blood and lymphatic vessels, and in bone marrow stem cells. ERG is expressed in virtually all endothelial neoplasms including hemangioendothelioma, angiosarcoma and Kaposi sarcoma. ERG is overexpressed secondary to gene rearrangement in cases of prostate adenocarcinoma, gastrointestinal stromal tumor, synovial sarcoma, meningioma, epithelioid sarcoma, malignant rhabdoid tumor, acute myeloid leukemia and blastic extramedullary myeloid tumor, and rarely Ewing sarcoma / primitive peripheral neuroectodermal tumor, chondrosarcoma, osteosarcoma, and rhabdomyosarcoma. For the identification of endothelial differentiation ERG seems more sensitive and specific than any other marker. Moreover, the interpretation is often easier due to the nuclear reaction, which also allows for double stains with cytoplasmic markers like podoplanin. Among carcinomas, ERG is highly specific for prostate, while the sensitivity is moderate.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa450 to C-terminus of ERG was used as an immunogen

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.Storage and StabilityAntibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ERG

Target: ERG (ERG, ETS transcription factor (ERG))

Alternative Name: ERG

Background:

ERG, ERG/EWS fusion gene, ERG/FUS fusion gene, ERG/TMPSSR2 fusion gene, ERG1, ETS-related, KCNH2, Oncogene ERG, p55, TMPRSS2/ERG fusion

Ewing sarcoma or prostate adenocarcinoma tissues., Human tonsil, Human Lung, Skin, A431

Molecular Weight: 55kDa

Gene ID: 2078, 473819

UniProt: P11308