PKC antibody (C-Term)

Catalog No. ABIN7860719

This Mouse Monoclonal antibody specifically detects PKC in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PKC antibody (C-Term) (ABIN7860719)

Target: PKC (Protein Kinase C (PKC))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 133

Product Details anti-PKC Antibody

Binding Specificity: C-Term

Purpose: Protein Kinase C α / PRKCA Antibody

Specificity: Protein Kinase C alpha (PKC°™) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the °™ isoform of PKC°™ and binds to a sequence at the C terminus of PKC°™ to detect its expression in vitro.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCα.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PKC

Target: PKC (Protein Kinase C (PKC))

Alternative Name: Protein Kinase C α

Background:

PKC alpha, PKC-A, PKC-alpha, PKCA, PRKCA, Protein kinase C alpha, Protein kinase C alpha type

Normal human kidney or tonsil tissue. HEK293 whole cell lysates.

Molecular Weight: 77kDa

Gene ID: 5578, 531704, 708867

UniProt: P17252

Pathways: TCR Signaling, Regulation of G-Protein Coupled Receptor Protein Signaling, Interaction of EGFR with phospholipase C-gamma, Phototransduction