Recombinant CD5 antibody (Extracellular Domain)

Catalog No. ABIN7864325

The Rabbit Monoclonal anti-CD5 antibody (Clone C5-8117R) (ABIN7864325) specifically detects CD5 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD5 antibody (Extracellular Domain) (ABIN7864325)

Target: CD5

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C5-8117R

Product Details anti-CD5 Antibody

Binding Specificity: Extracellular Domain

Purpose: Recombinant CD5 (Mantle Cell Lymphoma Marker) Antibody

Specificity: The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10 % of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20 % of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant protein corresponding to human extracellular domain of CD5 (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background:

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

MOLT-4, Jurkat or Raji cells. Human tonsil or lymph node.

Molecular Weight: 67kDa

Gene ID: 921, 58685

UniProt: P06127