CEBPE antibody

Catalog No. ABIN7864631

The Mouse Monoclonal anti-CEBPE antibody is suitable to detect CEBPE in samples from Human. It has been validated for IF and FACS.

Quick Overview for CEBPE antibody (ABIN7864631)

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEBPE antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-CEBPE-1G12

Product Details anti-CEBPE Antibody

Purpose: CEBPE Antibody

Specificity: The transcription factor C/EBP ± (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP ± regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP ± uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic ± helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP ± appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP ≤, C/EBP ≥, C/EBP t and C/EBP μ, all of which exhibit similar DNA-binding specificities and affinities to C/EBP ±. Furthermore, C/EBP ≤ and C/EBP t readily form heterodimers both with each other as well as with C/EBP ±.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CEBPE protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CEBPE

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Alternative Name: CEBPE

Background:

C/EBP epsilon, CCAAT/enhancer binding protein (C/EBP) epsilon, CCAAT/enhancer binding protein epsilon, CCAAT/enhancer-binding protein epsilon, CEBPE, CRP1

HeLa or MCF-7 cells.

Molecular Weight: 32/30/27/14kDa

Gene ID: 1053, 558308

Pathways: Cellular Response to Molecule of Bacterial Origin