CD16 antibody

Catalog No. ABIN7862809

The Mouse Monoclonal anti-CD16 antibody is suitable to detect CD16 in samples from Human. It has been validated for FACS, IF and WB.

Quick Overview for CD16 antibody (ABIN7862809)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: C16-1045

Product Details anti-CD16 Antibody

Purpose: CD16 / FcγReceptor III Antibody

Specificity: It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CD16 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background:

FcRIIIa, FCR-10, FcRIII, FCGRIII, FcR-10, Fc fragment of IgG, low affinity IIIa, receptor for (CD16), neutrophil-specific antigen NA, CD16a, low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIIIa, immunoglobulin G Fc receptor III, Fc-gamma RIII-alpha, Fc-gamma receptor IIIb (CD16), CD16a antigen, Fc-gamma receptor III-2 (CD16), FCGR3, Fc gamma receptor III-A, FCG3, low affinity III, receptor for (CD16), Fc-gamma RIII, IGFR3FcgammaRIIIA, CD16FCRIIIA, FCRIII, Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

hPBL or U-937 cells. Lymph nodes and tonsils., K-562. Human Liver. Human Lung. Human Spleen.

Molecular Weight: 50-80kDa

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process