Recombinant CD63 antibody

Catalog No. ABIN7861087

The Rabbit Monoclonal anti-CD63 antibody (Clone LAMP3-8770R) (ABIN7861087) specifically detects CD63 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD63 antibody (ABIN7861087)

Target: CD63

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: LAMP3-8770R

Product Details anti-CD63 Antibody

Purpose: Recombinant CD63 (Late Endosomes Marker) Antibody

Specificity: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CD63 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background:

gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30

HL60|SK-MEL-28|THP-1 or NIH/3T3 cells. Melanoma or lymphoma.|U87MG, Kidney, Lung, Pancreas

Molecular Weight: 26kDa (core protein), 30-60kDa (glycosylated)

Gene ID: 967, 445570

UniProt: P08962