Recombinant Complement C4d antibody

Catalog No. ABIN7863829

The Rabbit Monoclonal anti-Complement C4d antibody is suitable to detect Complement C4d in samples from Human. It has been validated for ELISA, IHC and WB.

Quick Overview for Recombinant Complement C4d antibody (ABIN7863829)

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Complement C4d antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Clone: C4D-9213R

Product Details anti-Complement C4d Antibody

Purpose: Recombinant Complement 4d (C4d) (Acute Humoral Rejection Marker) Antibody

Specificity: This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Complement 4d protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Alternative Name: Complement 4d

Background:

Acidic Complement C4, Basic C4, C4-1, C4a Anaphylatoxin, C4A, C4A2, C4A3, C4A4, C4A6, C4b, C4B1, C4B12, C4d fragment, C4F, C4S, Complement C4 Gamma Chain, Complement Component 4A, Complement Component 4B

Rejected Renal Transplant Tissue

Molecular Weight: 192kDa (predicted)

Gene ID: 720, 721

UniProt: P0C0L4, P0C0L5