CRYAB antibody

Catalog No. ABIN7863997

The Mouse Monoclonal anti-CRYAB antibody (Clone CRYAB-7918) (ABIN7863997) specifically detects CRYAB in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for CRYAB antibody (ABIN7863997)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CRYAB-7918

Product Details anti-CRYAB Antibody

Purpose: Crystallin Alpha B Antibody

Specificity: Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into ±, ≤ and ≥ families, and the ≤- and ≥-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide and N- and C-terminal extensions. ±-crystallins consist of three gene products, ±A-, ±B- and ±C-crystallin, which are members of the small heat shock protein family (HSP 20). ±-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, ±-crystallins do not renature these proteins. Expression of ±A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human ±B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of ±B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length CRYAB protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: Crystallin Alpha B

Background:

AACRYA, Alpha B crystallin, Alpha-crystallin B chain, CRYA2, CTTP2, Heat shock 20 kD like protein, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Rosenthal fiber component

Human heart, kidney or brain. HeLa or Y79 cells.

Molecular Weight: Predicted: 20kDa, Observed: 22-30kDa

Gene ID: 1410, 53454

UniProt: P02511