EIF2C3 antibody

Catalog No. ABIN7862722

The Mouse Monoclonal anti-EIF2C3 antibody is suitable to detect EIF2C3 in samples from Human. It has been validated for ELISA, IF and FACS.

Quick Overview for EIF2C3 antibody (ABIN7862722)

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2C3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-AGO3-1C5

Product Details anti-EIF2C3 Antibody

Purpose: AGO3 (Argonaute-3) / eIF2C3 Antibody

Specificity: This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as argonaute RISC catalytic component 3.Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human AGO3 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EIF2C3

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Alternative Name: AGO3

Background:

argonaute 3, Argonaute3, eIF 2C 3, eIF-2C 3, eIF2C 3, Eif2c3, EIF2C3 protein, Eukaryotic translation initiation factor 2C 3, Eukaryotic translation initiation factor 2C3, FLJ12765, hAgo3, MGC86946, Protein argonaute-3

HeLa, MCF7 or U-2OS cells.

Molecular Weight: 97.36kDa

Gene ID: 192669, 657659

UniProt: Q9H9G7

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway