GCLM antibody

Catalog No. ABIN7861362

The Mouse Monoclonal anti-GCLM antibody is suitable to detect GCLM in samples from Human. It has been validated for IHC.

Quick Overview for GCLM antibody (ABIN7861362)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: GCLM-4068

Product Details anti-GCLM Antibody

Purpose: GCLM (DNA Mismatch Repair Protein) Antibody

Specificity: G-glutamylcysteine synthetase (g-GCS) is the rate limiting enzyme for glutathione (L-g-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. g-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human g-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human g-GCS gene encoding the regulatory subunit maps to chromosome 1p22.1. The two subunits of g-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human GCLM protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background:

Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GCLM, GCS light chain, GLCLR, Glutamate--cysteine ligase modifier subunit, Glutamate--cysteine ligase regulatory subunit, GSC light chain

Human colon carcinoma (IHC).

Molecular Weight: 31kDa

Gene ID: 2730, 315562