HES5 antibody

Catalog No. ABIN7861398

The Mouse Monoclonal anti-HES5 antibody is suitable to detect HES5 in samples from Human. It has been validated for IHC.

Quick Overview for HES5 antibody (ABIN7861398)

Target: HES5 (Hairy and Enhancer of Split 5 (HES5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HES5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HES5-8035

Product Details anti-HES5 Antibody

Purpose: HES5 / bHLHb38 / Hes family bHLH transcription factor 5 Antibody

Specificity: The Drosophila Hairy and Enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila Hairy and Enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human HES5 protein (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HES5

Target: HES5 (Hairy and Enhancer of Split 5 (HES5))

Alternative Name: HES5

Background: BHLHb38

Molecular Weight: 41kDa

Gene ID: 388585, 57971

UniProt: Q5TA89

Pathways: Stem Cell Maintenance, Tube Formation