Recombinant HSP90 antibody

Catalog No. ABIN7864933

The Mouse Monoclonal anti-HSP90 antibody (Clone rHSPB1-7298) (ABIN7864933) specifically detects HSP90 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant HSP90 antibody (ABIN7864933)

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP90 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RHSPB1-7298

Product Details anti-HSP90 Antibody

Purpose: Recombinant Heat Shock 27kDa Protein 1 Antibody

Specificity: HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian ±-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length HSP27 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Alternative Name: Heat Shock Protein 1

Background:

Heat shock 27 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25 kDa protein 1, Heat shock 28 kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

MCF-7 or HeLa cells. ~50 % of breast carcinomas are positive for hsp27 especially those that are ER and/or PgR positive, Human Liver

Molecular Weight: 27kDa

Gene ID: 3315, 520973

UniProt: P04792

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals