HDAC1 antibody

Catalog No. ABIN7862935

The Mouse Monoclonal anti-HDAC1 antibody is suitable to detect HDAC1 in samples from Human. It has been validated for ELISA, IF and FACS.

Quick Overview for HDAC1 antibody (ABIN7862935)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-HDAC1-1B7

Product Details anti-HDAC1 Antibody

Purpose: Histone Deacetylase 1 (HDAC1) Antibody

Specificity: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/ CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human HDAC1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: Histone Deacetylase 1

Background:

DKFZp686H12203, GON 10, HD1, Reduced potassium dependency yeast homolog like 1 (RPD3L1), RPD3

HeLa or Jurkat cells.

Molecular Weight: 60kDa

Gene ID: 3065, 88556

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis