MXI1 antibody

Catalog No. ABIN7863173

The Mouse Monoclonal anti-MXI1 antibody is suitable to detect MXI1 in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for MXI1 antibody (ABIN7863173)

Target: MXI1 (MAX Interactor 1 (MXI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MXI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-MXI1-1A3

Product Details anti-MXI1 Antibody

Purpose: MXI1 Antibody

Specificity: It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (also designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad- binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MXI1 protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MXI1

Target: MXI1 (MAX Interactor 1 (MXI1))

Alternative Name: MXI1

Background:

bHLHc11, Class C basic helix-loop-helix protein 11, MAX dimerization protein 2 (MAD2), MAX interacting protein 1, Max interactor 1, Max related transcription factor, MXD2, MXI, MXI1 protein

HeLa or K562 cells.

Molecular Weight: 26/22/33/21 kDa (isoforms)

Gene ID: 4601

UniProt: P50539

Pathways: Maintenance of Protein Location