MED7 antibody

Catalog No. ABIN7863632

This Mouse Monoclonal antibody specifically detects MED7 in ELISA, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for MED7 antibody (ABIN7863632)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED7 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PCRP-MED7-1B8

Product Details anti-MED7 Antibody

Purpose: MED7 Antibody

Specificity: MED7, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MED7 protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: MED7

Background:

Activator recruited cofactor 34 kDa component, ARC34, Cofactor required for Sp1 transcriptional activation, subunit 9, 33 kDa, CRSP complex subunit 9, CRSP9, hMED7, Mediator of RNA polymerase II transcription subunit 7, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33

HeLa, U-251 MG or CACO-2 cells.

Molecular Weight: 27.2kDa

Gene ID: 9443, 279902

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha