Recombinant MLANA antibody

Catalog No. ABIN7861713

The Mouse Monoclonal anti-MLANA antibody is suitable to detect MLANA in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant MLANA antibody (ABIN7861713)

Target: MLANA (Melan A (MLANA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RMLANA-9404

Product Details anti-MLANA Antibody

Purpose: Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody

Specificity: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MART-1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MART-1

Background:

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

SK-MEL-13 or SK-MEL-19 melanoma cell lines. Melanomas, COLO 38 cells

Molecular Weight: 20-22kDa (doublet)

Gene ID: 2315, 154069

UniProt: Q16655