NMI antibody

Catalog No. ABIN7863575

This Mouse Monoclonal antibody specifically detects NMI in FACS. It exhibits reactivity toward Human.

Quick Overview for NMI antibody (ABIN7863575)

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NMI antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-NMI-1C1

Product Details anti-NMI Antibody

Purpose: N myc (and STAT) Interactor / NMI Antibody

Specificity: Nmi (for N-Myc interactor) is an interferon inducible protein that associates with multiple transcription factors, including c-Myc, n-Myc, Max, and c-Fos, which contain bHLH-ZIP, bHLH, or Zip domains. Nmi is ubiquitously expressed at low levels throughout various fetal and adult tissues and at higher levels in myeloid leukemias and cell lines overexpressing c-Myc. In addition to binding Myc proteins, Nmi also associates with the Stat family of transcription factors, where it enhances Stat-dependent transcription. Although Nmi lacks an intrinsic DNA binding or activation domain, Nmi enhances the transcriptional activity of the Stat proteins, in response to cytokine stimulation, by recruiting the Stat1 and Stat5 transcriptional coactivators, CREB-binding protein (CBP) and p300. In vitro studies indicate that Nmi, expressed in conjunction with CBP, enhances the transcriptional responsiveness of Stat5 to IL-2 stimulation five-fold over CBP alone by increasing the affinity of Stat proteins for CBP/p300.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human NMI protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: N myc Interactor

Background:

N myc (and STAT) interactor, N myc interactor, NMI, NMYC and STAT interactor, NMYC interactor

HeLa or K562 cells.

Molecular Weight: 38kDa

Gene ID: 9111, 54483

UniProt: Q13287