NFIA antibody

Catalog No. ABIN7863189

This Mouse Monoclonal antibody specifically detects NFIA in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFIA antibody (ABIN7863189)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-NFIA-2C6

Product Details anti-NFIA Antibody

Purpose: NFIA / NF1A (Nuclear Factor 1A) (Transcription Factor) Antibody

Specificity: NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, °™-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human NFIA protein corresponding to protein domain

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA

Background:

CCAAT-box-binding transcription factor, CTF, NF I/A, NF-I/A, NF1-A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1 A-type, TGGCA binding protein

Colon, HeLa cells. Human fetal gut, liver or heart.

Molecular Weight: 55kDa

Gene ID: 4774, 322431

UniProt: Q15784