NME2 antibody

Catalog No. ABIN7861862

This Mouse Monoclonal antibody specifically detects NME2 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for NME2 antibody (ABIN7861862)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NME2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: NME2-6434

Product Details anti-NME2 Antibody

Purpose: NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis) Antibody

Specificity: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human NME2 protein

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Alternative Name: NME2

Background:

C myc purine binding transcription factor PUF, C-myc purine-binding transcription factor PUF, epididymis secretory sperm binding protein Li 155an, HEL-S-155an, Histidine protein kinase NDKB, NDP kinase B, NDPKB, nm23-H2, NM23B, NME/NM23 nucleoside diphosphate kinase 2, nme2, Nucleoside diphosphate kinase B,

293T, MCF7 Ubiquitously expressed in all tissues.

Molecular Weight: 17kDa

Gene ID: 4831, 463456

UniProt: P22392