Recombinant pan Keratin antibody

Catalog No. ABIN7863730

This Mouse Monoclonal antibody specifically detects pan Keratin in IF, IHC, FACS and WB. It exhibits reactivity toward Human, Dog and Cat.

Quick Overview for Recombinant pan Keratin antibody (ABIN7863730)

Target: pan Keratin (panKRT)

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Clone: Cocktail rPCK-6750

Product Details anti-pan Keratin Antibody

Purpose: Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

Specificity: Anti-cytokeratin clone rPCK/6750 (CK rPCK/6750) demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin, pan

Background:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

adeno- or squamous carcinomas (IHC)., Skin, A431.

Molecular Weight: 40-67kDa ()