PPARD antibody

Catalog No. ABIN7864409

The Mouse Monoclonal anti-PPARD antibody is suitable to detect PPARD in samples from Human. It has been validated for FACS.

Quick Overview for PPARD antibody (ABIN7864409)

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPARD antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-PPARD-4C2

Product Details anti-PPARD Antibody

Purpose: PPARD Antibody

Specificity: Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARa, PPARb (also designated PPARé 79oΩ) and PPARg, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARa is abundant in primary hepatocytes, where it regulates the expression of proteins involved in fatty acid metabolism. PPARb is the most widely distributed subtype and is often expressed at high levels. PPARg is predominantly seen in adipose tissue, where it plays a critical role in regulating adipocyte differentiation. Interestingly, both the orphan nuclear hormone receptor LXRa and thyroid receptor (TR) have been shown to act as antagonists of PPARa/ RXRa binding to PPREs.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around 72-146) of human PPARD protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for PPARD

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Alternative Name: PPARD

Background:

FAAR, NR1C2, NUC1, NUCII

HeLa or MCF-7 cells.

Molecular Weight: 49.9kDa

Gene ID: 5467, 696032

UniProt: Q03181

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation