PRDM1 antibody

Catalog No. ABIN7864854

The Mouse Monoclonal anti-PRDM1 antibody is suitable to detect PRDM1 in samples from Human and Mouse. It has been validated for FACS and IF.

Quick Overview for PRDM1 antibody (ABIN7864854)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PCRP-PRDM1-2B9

Product Details anti-PRDM1 Antibody

Purpose: PRDM1 / BLIMP-1 Antibody

Specificity: The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-Myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human PRDM1 protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1

Background:

B Lymphocyte Induced Maturation Protein 1 , Beta-interferon gene positive regulatory domain I-binding factor , BLIMP1 , Positive Regulatory Domain I Binding Factor 1 , PR domain containing 1 with ZNF domain isoform 2 , PR domain zinc finger protein 1 , PR domain-containing protein 1 , PRDI-BF1 , PRDI-binding factor 1

HeLa or MCF-7 cells.

Molecular Weight: 114.8kDa

Gene ID: 639, 436023

Pathways: Regulation of Muscle Cell Differentiation