PBX2 antibody

Catalog No. ABIN7863207

The Mouse Monoclonal anti-PBX2 antibody is suitable to detect PBX2 in samples from Human. It has been validated for ELISA, IF and FACS.

Quick Overview for PBX2 antibody (ABIN7863207)

Target: PBX2 (Pre-B-Cell Leukemia Homeobox Protein 2 (PBX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PBX2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-PBX2-1C4

Product Details anti-PBX2 Antibody

Purpose: PBX2 / PRL (Transcription Factor) Antibody

Specificity: Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1,19)(q23,p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92 % and 94 % respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human PBX2 protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PBX2

Target: PBX2 (Pre-B-Cell Leukemia Homeobox Protein 2 (PBX2))

Alternative Name: PBX2

Background:

G17, Homeobox protein PBX2, HOX12, PBX2MHC, pre B cell leukemia homeobox 2, Pre-B-cell leukemia transcription factor 2, Protein G17

HeLa cells. Expressed in all tissues except in cells of the B and T lineage.

Molecular Weight: 46kDa

Gene ID: 5089, 557097

UniProt: P40425

Pathways: Skeletal Muscle Fiber Development