QKI antibody

Catalog No. ABIN7863633

The Mouse Monoclonal anti-QKI antibody is suitable to detect QKI in samples from Human. It has been validated for WB, IF, ELISA and FACS.

Quick Overview for QKI antibody (ABIN7863633)

Target: QKI (Protein Quaking (QKI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This QKI antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Clone: PCRP-QKI-2F10

Product Details anti-QKI Antibody

Purpose: QKI / Protein Quaking Antibody

Specificity: QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human QKI protein

Isotype: IgG2c

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for QKI

Target: QKI (Protein Quaking (QKI))

Alternative Name: QKI

Background:

Homolog of mouse quaking QKI KH domain RNA binding protein, HQK1, Protein quaking, QK1, QK3, QKI, QKI1, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

HeLa, K562 or MCF7 cells., U87

Molecular Weight: 37.6kDa

Gene ID: 9444, 510324

UniProt: Q96PU8