SPIC antibody

Catalog No. ABIN7862651

The Mouse Monoclonal anti-SPIC antibody is suitable to detect SPIC in samples from Human. It has been validated for IF and FACS.

Quick Overview for SPIC antibody (ABIN7862651)

Target: SPIC (Spi-C Transcription Factor (Spi-1/PU.1 Related) (SPIC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPIC antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-SPIC-2C5

Product Details anti-SPIC Antibody

Purpose: SPI-C / Transcription factor Spi-C Antibody

Specificity: The Ets transcription factor family is comprised of DNA-binding proteins that influence lymphoid development and activity and bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. Spi-B and Spi-C (also known as SPIC) are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to non-canonical AGAA sites. Spi-C is a 248 amino acid protein that localizes to the nucleus and, like other Ets family members, binds DNA as a monomer and plays a role in transcriptional regulation. Addi- tionally, Spi-C is thought to control the development of red pulp macrophages, thereby contributing to iron homeostasis and red blood cell recycling. Human Spi-C shares 65 % amino acid identity with its mouse counterpart, suggesting a conserved role between species.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SPIC protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SPIC

Target: SPIC (Spi-C Transcription Factor (Spi-1/PU.1 Related) (SPIC))

Alternative Name: SPI-C

Background:

AU019198, C76795, MGC40611, MGC80555, Prf, Pu.1 related factor, Spi C, Spi C transcription factor, Spi-C transcription factor (Spi-1/PU.1 related)

Jurkat or Raji cells.

Molecular Weight: 29kDa

Gene ID: 121599, 577097

UniProt: Q8N5J4