TDP2 antibody

Catalog No. ABIN7863224

The Mouse Monoclonal anti-TDP2 antibody is suitable to detect TDP2 in samples from Human. It has been validated for IHC, IF and FACS.

Quick Overview for TDP2 antibody (ABIN7863224)

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TDP2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TDP2-1258

Product Details anti-TDP2 Antibody

Purpose: TDP2 / EAPII Antibody

Specificity: This MAb recognizes a protein of 41 kDa, which is identified as TDP2. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human TDP2 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TDP2

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Alternative Name: TDP2

Background:

5'-Tyr-DNA phosphodiesterase, EAP2, EAPII, ETS1-associated protein 2, ETS1-associated protein II, TRAF and TNF receptor-associated protein, TTRAP, Tyr-DNA phosphodiesterase 2, Tyrosyl-DNA phosphodiesterase 2 (TDP2)

Duodenum or Small Intestinal Carcinoma., Jurkat cells. Lung

Molecular Weight: ~41kDa

Gene ID: 51567, 728795

UniProt: O95551