Recombinant Ubiquitin antibody

Catalog No. ABIN7865473

The Mouse Monoclonal anti-Ubiquitin antibody (Clone rFPM1) (ABIN7865473) specifically detects Ubiquitin in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Ubiquitin antibody (ABIN7865473)

Target: Ubiquitin (Ub)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RFPM1

Product Details anti-Ubiquitin Antibody

Purpose: Recombinant Ubiquitin (Autophagy Marker) Antibody

Specificity: Ubiquitin, a small protein consisting of 76 amino acids, has been found in all eukaryotic cells studied. It is one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation, which eliminates most intracellular defective proteins as well as normal proteins with a rapid turnover. Degradation involves covalent binding of ubiquitin to the protein to be degraded and it is believed that in this way ubiquitin acts to label the protein for disposal by intracellular proteases. The most abundant ubiquitin-protein conjugate, however, is ubiquitin-histone H2A. This conjugate is not degraded. Since such ubiquitinated histones are present primarily in actively transcribed chromosomal regions, ubiquitin may play a role in regulation of gene expression.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Ubiquitin conjugated with glutaraldehyde crosslinked to keyhole limpet hemocyanin (KLH).

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background:

Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B

HeLa or Raji cells. Human brain.

Molecular Weight: 9kDa

Gene ID: 7314, 356190, 520348

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway