WTAP antibody

Catalog No. ABIN7865027

The Mouse Monoclonal anti-WTAP antibody is suitable to detect WTAP in samples from Human. It has been validated for IHC and FACS.

Quick Overview for WTAP antibody (ABIN7865027)

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WTAP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: PCRP-WTAP-1A4

Product Details anti-WTAP Antibody

Purpose: WTAP Antibody

Specificity: Wilms tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human WTAP protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for WTAP

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WTAP

Background:

Female-lethal(2)D homolog, hFL(2)D, PNAS132, Pre-mRNA-splicing regulator WTAP, Putative pre mRNA splicing regulator female lethal(2D), Wilms' tumour 1-associating protein, WT1 associated protein

Ubiquitous expression.

Molecular Weight: 44.2kDa

Gene ID: 9589, 446091

UniProt: Q15007