DNA-RNA Hybrid antibody

Catalog No. ABIN7865241

The Mouse Monoclonal anti-DNA-RNA Hybrid antibody is suitable to detect DNA-RNA Hybrid in samples from Various Species and Human. It has been validated for ChIP, ICC, IF, FACS and IP.

Quick Overview for DNA-RNA Hybrid antibody (ABIN7865241)

Target: DNA-RNA Hybrid

Reactivity: Various Species, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: S9-6

Product Details

Purpose: DNA-RNA Hybrid Antibody

Specificity: We have not tested this antibody in-house in Immunofluorescence, CHIP, Immunocytochemistry, Immunoprecipitation or Flow Cytometry. All application recommendations come from publications using this clone. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate.

Immunogen: DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details

Target: DNA-RNA Hybrid

Background:

DNA-RNA Duplex, DNA/RNA Duplex, DNA:RNA Duplex, DNA-RNA Hybrid, DNA/RNA Hybrid, DNA:RNA Hybrid, RNA-DNA Duplex, RNA/DNA Duplex, RNA:DNA Duplex, RNA-DNA Hybrid, RNA/DNA Hybrid, RNA:DNA Hybrid

All. Target DNA-RNA heteroduplex (R loop) structure is not sequence-specific or species-specific.