Recombinant FLI1 antibody

Catalog No. ABIN7862396

This Rabbit Monoclonal antibody specifically detects FLI1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant FLI1 antibody (ABIN7862396)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: FLI1-4371R

Product Details anti-FLI1 Antibody

Purpose: Recombinant FLI1 (Ewing s Sarcoma & Endothelial Marker) Antibody

Specificity: Recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Purification: 200ug/ml of Ab purified from rabbit anti-serum by Protein A.

Immunogen: Recombinant full-length human FLI1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background:

ERGB transcription factor, Ewing Sarcoma breakpoint region 2 (EWSR2), FLI1, FLI1 EWS fusion gene, Friend leukemia integration 1 (FLI1) transcription factor, Friend leukemia virus integration 1, Proto-oncogene Fli-1, SIC1, Transcription factor ERGB, Viral integration region FLI1

Ewing s sarcoma (EWS) or angiosarcoma.|THP1 or Raji cells. Human tonsil

Molecular Weight: 51kDa

Gene ID: 2313, 504281

UniProt: Q01543