Ubiquitin antibody (acLys29)

Catalog No. ABIN7862470

The Rabbit Polyclonal anti-Ubiquitin antibody (ABIN7862470) specifically detects Ubiquitin in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Ubiquitin antibody (acLys29) (ABIN7862470)

Target: Ubiquitin (Ub)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ubiquitin Antibody

Binding Specificity: acLys29

Purpose: Rabbit Anti-Ub (Acetyl Lys29) Polyclonal Antibody

Specificity: Acetyl-Ub (K29) Polyclonal AntibodySynthesized peptide derived from the human Ub around the acetylation site of K29.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the human Ub around the acetylation site of K29

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ub

Background: UBB, Polyubiquitin-B, UBC, Polyubiquitin-C, RPS27A, UBA80, UBCEP1, Ubiquitin-40S ribosomal protein S27a, Ubiquitin carboxyl extension protein 80, UBA52, UBCEP2, Ubiquitin-60S ribosomal protein L40, CEP52, Ubiquitin A-52 residue ribosomal protein fusion prUbiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27.

Molecular Weight: 80kD

UniProt: P62987, P62979, P0CG47, P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway