Obestatin antibody

Catalog No. ABIN7862539

The Rabbit Polyclonal anti-Obestatin antibody (ABIN7862539) specifically detects Obestatin in ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Obestatin antibody (ABIN7862539)

Target: Obestatin (OB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Obestatin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Obestatin Antibody

Purpose: Rabbit Anti-Ob Polyclonal Antibody

Specificity: The antibody detects endogenous levels of Ob

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human Ob protein at AA range: 10-50

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-200,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Obestatin

Target: Obestatin (OB)

Alternative Name: Ob

Background: Leptin, Obese protein, Obesity factorLEP encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations inLEP and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in LEP has also been linked to type 2 diabetes mellitus development. LEP (Leptin) is a Protein Coding gene. Diseases associated with LEP include Obesity, Morbid, Due To Leptin Deficiency and Obesity Due To Congenital Leptin Deficiency. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and Transcription factor regulation in adipogenesis.

Gene ID: 3952

UniProt: P41159