alpha Fetoprotein antibody

Catalog No. ABIN7865556

The Mouse Monoclonal anti-alpha Fetoprotein antibody is suitable to detect alpha Fetoprotein in samples from Human, Pig, Dog and Monkey. It has been validated for ELISA, IHC, IF, FACS and ICC.

Quick Overview for alpha Fetoprotein antibody (ABIN7865556)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Pig, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: C3

Product Details anti-alpha Fetoprotein Antibody

Purpose: anti-Alpha fetoprotein (AFP) mouse monoclonal, C3, purified

Cross-Reactivity (Details): not with cow, mouse, rat

Purification: affinity chromatography

Immunogen: alpha fetoprotein isolated from serum of hepatoma patients

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: Alpha fetoprotein (AFP)

Background: C3 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nunstad to a group of antibodies with low affinity to human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa and expressed in fetal liver but normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some other germ cell tumors and in hepatocellular carcinomas. Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.

Pathways: C21-Steroid Hormone Metabolic Process