CD16 antibody

Catalog No. ABIN7865576

This Mouse Monoclonal antibody specifically detects CD16 in FACS, IF, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for CD16 antibody (ABIN7865576)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: ICO-116

Product Details anti-CD16 Antibody

Purpose: anti-CD16 mouse monoclonal, ICO-116, purified

Purification: affinity chromatography

Immunogen: human PBL

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: ICO-116 specifically recognizes CD16. This molecule also named low affinity Fc receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. ICO-116 binds to 15 % peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity. Positive control: K-562 or U-937 cells. Lymph nodes and tonsils.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process