CD27 antibody

Catalog No. ABIN7865583

The Mouse Monoclonal anti-CD27 antibody is suitable to detect CD27 in samples from Human. It has been validated for FACS, WB, IHC, IF and ICC.

Quick Overview for CD27 antibody (ABIN7865583)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 203-6

Product Details anti-CD27 Antibody

Purpose: anti-CD27 mouse monoclonal, 203-6, purified

Purification: affinity chromatography

Immunogen: Sézary cells

Isotype: IgG3 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: 203-6 Reacts with human CD27, a disulphide-linked 120 kDa dimer. CD27 is a lymphocyte-specific member of the TNF-R/NGF-R superfamily, and is expressed on a subset of human thymocytes and on the majority of mature T-lymphocytes. CD27 is highly expressed on activated T and B-cells. 203-6 was clustered at the VIth WLDA. Positive control: Human PBL or tonsil.

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints