CD53 antibody

Catalog No. ABIN7865598

The Mouse Monoclonal anti-CD53 antibody is suitable to detect CD53 in samples from Human. It has been validated for FACS, IF, IHC and ICC.

Quick Overview for CD53 antibody (ABIN7865598)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 63-5A3

Product Details anti-CD53 Antibody

Purpose: anti-CD53 mouse monoclonal, 63-5A3, purified

Purification: affinity chromatography

Immunogen: human Sezary cells

Isotype: IgG2b kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: CD53 is a 33-55 kDa protein expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on B- an T-cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. 161-2 was typed in Kobe, Japan at the VIth International Workshop on human leucocyte differentiation antigens. Positive control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes, human tonsil and lymph node.