CD63 antibody

Catalog No. ABIN7865601

The Mouse Monoclonal anti-CD63 antibody is suitable to detect CD63 in samples from Human and Mouse. It has been validated for FACS, IHC, IF and ICC.

Quick Overview for CD63 antibody (ABIN7865601)

Target: CD63

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: EBS-CD-036

Product Details anti-CD63 Antibody

Purpose: anti-CD63 mouse monoclonal, EBS-CD-036, purified

Purification: affinity chromatography

Immunogen: MeWo cell membranes

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

IHC-frozen_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: EBS-CD-036 reacts with CD63 and is mainly used in combination with EBS-CD-147 and/or anti-PEM (MUC1) to identify melanoma from carcinoma in paraffin sections. Melanomas are EBS-CD-036 and EBS-CD-147 positive, but PEM negative. EBS-CD-036 reacts in frozen sections with melanoma and breast cancers, smooth muscle and lung (weakly). In paraffin sections melanomas (primary skin, uveal and choroidal), melanoma metastases, clear cell CA, carcinoids, skin nevi, medullary CA of thyroid, prostate CA, some breast, ovary, lung, colorectal and bladder CA positive. Normal tissues that are positive include: mast cells, sweat glands, Islets of Langerhans, pituitary, pancreas, peribronchial glads, Paneth cells and prostate glands. Positive control: SK-MEL-28, HL60, THP-1, NIH/3T3 cells, Melanoma, lymphoma.