CD86 antibody

Catalog No. ABIN7865607

The Mouse Monoclonal anti-CD86 antibody is suitable to detect CD86 in samples from Human, Mouse and Rat. It has been validated for FACS, IHC, IF and ICC.

Quick Overview for CD86 antibody (ABIN7865607)

Target: CD86

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: EBS-CD-042

Product Details anti-CD86 Antibody

Purpose: anti-CD86 mouse monoclonal, EBS-CD-042, purified

Purification: affinity chromatography

Immunogen: ARH-77 cells (B-lymphoblastoid cell line)

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: EBS-CD-42 reacts with CD86, a member of the immunoglobulin superfamily, and highly expressed on monocytes, dendritic cells and stimulated B-cells. It is probably the major CD28 ligand. Furthermore, EBS-CD-042 blocks binding of soluble CD152 to CD86. Positive control: 293T or Jurkat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation