CDw17 antibody

Catalog No. ABIN7865612

This Mouse Monoclonal antibody specifically detects CDw17 in FACS, IF, ICC and IHC. It exhibits reactivity toward Human.

Quick Overview for CDw17 antibody (ABIN7865612)

Target: CDw17

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Clone: EBS-CD-014

Product Details

Purpose: anti-CDw17 mouse monoclonal, EBS-CD-014, purified

Purification: affinity chromatography

Immunogen: beta-2 microglobulin associated proteins from a detergent lysate of human PBL

Isotype: IgM kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details

Target: CDw17

Background: CDw17 is an intermediate glycosphingolipid from the metabolism of higher gangliosides that localizes to sphingolipid-sterol rafts. CDw17 is found on monocytes, granulocytes, basophils, platelets, a subset of peripheral B-cells (CD19+) and tonsil dendritic cells. It is rapidly down regulated on activated granulocytes and is upregulated on IL-2 activated T-lymphocytes. CDw17 binds to bacteria and may function in phagocytosis. It may also be involved in angiogenesis. Aberrant levels of glycosphingolipids are a feature of cancer cells and may influence integrin clustering and internalization. Positive control: Human PBL and tonsil.