CCL20 antibody

Catalog No. ABIN7865614

The Mouse Monoclonal anti-CCL20 antibody (Clone EBS-CD-027) (ABIN7865614) specifically detects CCL20 in IHC, ICC, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CCL20 antibody (ABIN7865614)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL20 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: EBS-CD-027

Product Details anti-CCL20 Antibody

Purpose: anti-CD45R0 mouse monoclonal, EBS-CD-027, purified

Purification: affinity chromatography

Immunogen: lentil lectin purified glycoproteins from leucocytes from a B-cell CCL patient

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CD45R0

Background: CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). EBS-CD-027 reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B-cells or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemia's. It rarely stains NK-cells or B-cell lymphomas. Positive control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in tonsil or reactive lymph node.

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection